Journal: Scientific Reports

Article Title: Umbilical cord tissue is a robust source for mesenchymal stem cells with enhanced myogenic differentiation potential compared to cord blood

doi: 10.1038/s41598-020-75102-9

Figure Lengend Snippet: Conversion of uMSCs into myogenic lineage. ( A ) Myogenic differentiation was induced by culturing UCB MSCs in myogenic differentiation medium M1 for 3, 6, and 10 days to detect Pax7, MyoD, Myogenin, and MyHC by ( i ) FACS and by ( ii ) immunocytochemistry. Nuclei were counterstained with DAPI. ( iii ) Myf5 mRNA expression in control and differentiated UCB MSCs after 3 days (* p = 0.03). ( iv ) Comparative immunofluorescent analysis of Pax7 and MyoD at 3 days, Myogenin at 7 days and MyHC at 10 days between UCT and UCB MSCs. UCT MSCs show increased expression of MyoD, Myogenin, and MyHC expressing cells compared to UCB MSCs at their corresponding time points (* p = 0.03). ( B ) Myogenin and MyHC mRNA expression in control UCT MSCs or UCT MSCs induced to differentiate in M1 medium with enhanced adhesion or M2 medium for 10 and 20 days (** p = 0.002). Robust expression of MyHC was observed in M1 medium with enhanced adhesion at 10 and 20 days.

Article Snippet: Further propagation of UCB and UCT derived MSCs was attained by plating in ɑ-MEM (Hyclone, cat # SH30568.FS) with 2 mM sodium pyruvate, 4 mM L-glutamine, 20% FBS, and antibiotics (henceforth referred to as growth medium), with the exception that UCB-derived MSCs were plated in growth medium supplemented with 1 ml of MesenPro growth supplement (ThermoFisher Scientific, cat # 12746012).

Techniques: Immunocytochemistry, Expressing